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Luminex液相抗体芯片

首页 » 研究 2016-11-24 R&D Systems 赞(2)
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ELISA实验能够很好的对蛋白进行精确定量且操作简单,然而需要同时检测多个样本的多个因子时,ELISA需要多次重复相同的步骤,不能很快的得到实验结果。Lumimex液相芯片,以ELISA三明治夹心原理,利用微球和基于流式细胞仪原理的Lumimex仪器相结合的技术,采用ELISA板的形式实现多样本验证,在单孔中同时检测多个蛋白指标,进行精确定量。

ELISA实验能够很好的对蛋白进行精确定量且操作简单,然而需要同时检测多个样本的多个因子时,ELISA需要多次重复相同的步骤,不能很快的得到实验结果。Lumimex液相芯片,以ELISA三明治夹心原理,利用微球和基于流式细胞仪原理的Lumimex仪器相结合的技术,采用ELISA板的形式实现多样本验证,在单孔中同时检测多个蛋白指标,进行精确定量。

Luminex液相抗体芯片技术采用不同颜色编码的微球,包被相应的特异性抗体,在一个孔可同时检测多达100种分析物,利用96孔板作为反应板,实现高通量的蛋白精确定量。R&D Systems公司有15年Luminex试剂盒的研发历史,具有丰富的免疫检测经验,采用与ELISA试剂盒质量一致的抗体对和缓冲液体系优化,在“金标准ELISA”的基础上,提供最高标准和最优性能的Luminex多重检测试剂,对多个生物标志物进行定量和筛选。

应用

实验原理

在充血性心力衰竭疾病患者体内,进一步优化生物标志物组合,检测其中 6 种指标。选取充血性心力衰竭病人 10 个,健康人 20 个,利用 Luminex High Performance Human Cardiac Panel A (货号 LUCA000) 检测 TNF RII/TNFRSF1B、ST2/IL-1 R4、CD40 Ligand、FailureProprotein Convertase 9/PCSK9、GDF-15 和 PAPP-A,其中 PAPP-A 在所有样本中浓度低于最低检测限。

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文献

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